The RNA Research Foundation
RNA Engineering to Improve Health
Nucline: A gene responsive RNA
Nucline is an RNA molecule that recognizes
genetic signals and then responds in
predetermined manners, such as making a
new protein. Nucline can perform complex
logic operations sensing combinations of
genetic sequences and specific RNA
concentrations within cells.
Programmable Cells (tm)
Nucline can be used to reprogram a cell to
act in new therapeutic ways. It can be used
to introduce new signals such as telling a
cancer cell or viral infected cell to kill itself,
or even tell a cell to stop making a defective
protein and instead make the correct one.
Patents and
Switching ON gene therapy
June 2003
The article descibes Nucline technology and many
of it uses.
U.S. Patent No. 6,323,003
Filed Dec. 20, 1999
The RNA Research Foundation promotes and develops RNA construct systems which
regulate cell function.

Our mission is to translate these constructs into viable therapeutics and agricultural
products to improve human and animal health.

One area of intense research is Nucline 5-3, which regulates cells using Boolean logic
much like a computer program. When Nucline is in a cell, the input is the cell's own
gene expression profile, while the output is a biological function such as shutting the
cell down or having it make a therapeutic protein. Nucline has proved to successfully
reprogram breast cancer cells in vitro.

Although the term "Riboregulators" post-dates the invention of Nucline, it belongs to
the same class of RNA molecules that regulate gene expression, including gene-sensing
RNA, riboswitchs, and cis-RNA regulators. These type of RNA constructs have become
the basis for an entirely new area of biology call "Synthetic Biology" where cells are
reprogrammed to operate according to the scientist's directions.

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The RNA Research Foundation

U.S. Patent No. 7,052,903
Filed Sep. 20, 2001
U.S. Patent No. 7,439,059
Filed Feb. 27, 2006